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Functional characterization of an (R)-selective styrene monooxygenase from streptomyces sp. NRRL S-31.

Enzyme Microb Technol. 2020; 
Cui C1, Guo C2, Lin H3, Ding ZY1, Liu Y2, Wu ZL4.
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DNA Sequencing … sp. NRRL S-31 [33]. The DNA sequence of StStyA was optimized for Escherichia<br> coli BL21 (DE3) expression (See Supporting information for optimized sequence),<br> and synthesized by <b>GenScript</b> Biotech Co. (Nanjing, China) … Get A Quote

摘要

Styrene monooxygenases (SMOs) are two-component enzymes known to catalyze the epoxidation of styrene to (S)-styrene oxide. In this work, we identified a new oxygenase component, named StStyA, from the genome of Streptomyces sp. NRRL S-31. StStyA displayed complementary stereoselectivity to all of the known SMOs when coupled with a known reductase component (PsStyB), which made it the first natural SMO that produces (R)-styrene oxide. Accordingly, a plasmid co-expressing StStyA and PsStyB was constructed, which led to an artificial two-component SMO, named StStyA/B. When applied in the bio-epoxidation of nine aromatic alkenes, the enzyme showed activity toward five alkenes, and consistently displayed (R)-selecti... More

关键词

Asymmetric synthesis; Chiral epoxide; Epoxidation; Monooxygenase; Styrene oxide