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Rapid detection and differentiation of Theileria annulata, T. orientalis and T. sinensis using high-resolution melting analysis.

Ticks Tick Borne Dis. 2020;

Wang J1, Yang J1, Gao S1, Liu A1, Rashid M1, Li Y1, Liu Z1, Liu J1, Liu G1, Luo J1, Guan G2, Yin H3.

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	… cells. Three clones from each sample were selected and sequenced using BigDye<br> Terminator Mix (<b>GenScript</b>, Nanjing, China). Sequences were subjected to blast<br> analysis on NCBI database using the BLASTn program. Table …	Get A Quote

摘要

Bovine theileriosis, caused by protozoan parasites of the genus Theileria, presents with various clinical symptoms. In cattle, clinical presentations and outcomes of bovine theileriosis are closely correlated with the causative Theileria spp. Thus, accurate detection and discrimination of Theileria spp. are essential for epidemiological studies and for provision of clinical management strategies. High-resolution melting (HRM) analyses of two amplicons targeting the 18S rRNA indicated that T. annulata, T. orientalis, and T. sinensis isolated from China can be accurately detected and discriminated with the lowest detection limit of 1-10 copy numbers of plasmid bearing the 18S rRNA sequence. The approach was verif... More