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Enhancement of pyruvic acid production in Candida glabrata by engineering hypoxia-inducible factor 1.

Bioresour Technol. 2020; 
Luo Z1, Zeng W2, Du G3, Chen J4, Zhou J5.
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DNA Sequencing … are listed in Table 1. In brief, human HIF1α (GenBank accession no: 3091) and HIF1β (GenBank<br> accession no: 405) genes, containing Pro-Lys-Lys-Lys-Arg-Lys-Val amino acid sequence in their<br> N-terminal, were codon-optimized and synthesized by <b>GenScript</b> (Nanjing, China … Get A Quote

摘要

Dissolved oxygen (DO) supply plays essential roles in microbial organic acid production. Candida glabrata, as a dominant strain for producing pyruvic acid, principally converts glucose to pyruvic acid through glycolysis. However, this process relies excessively on high extracellular DO content. In this study, in combination with specific motif analysis of gene promoters, hypoxia-inducible factor 1 (HIF1) was engineered to improve the transcription level of some enzymes related to pyruvic acid synthesis under low DO level and directly led to increased pyruvic acid production and glycolysis efficiency. Moreover, the intracellular stability of HIF1 was further optimized from different aspects to maximize pyruvic a... More

关键词

HIF1; Low dissolved oxygen; Optimization of HIF1 stability; Pyruvic acid