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Knockdown of the aminopeptidase N genes decreases susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca.

Pestic Biochem Physiol. 2020; 
Sun Y1, Yang P1, Jin H1, Liu H1, Zhou H1, Qiu L2, Lin Y3, Ma W4.
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DNA Sequencing … Correct inserts of the recombinant plasmids were confirmed by sequencing conducted by the<br> <b>GenScript</b> Biology Company, Nanjing, China. For dsRNA expression, 200 ng of recombinant<br> plasmids were transformed into competent Escherichia coli HT115 (DE3) cells … Get A Quote

摘要

Bacillus thuringiensis (Bt) insecticide is currently the most widely used bioinsecticide. Bt expressing cry genes are some of the most successful foreign-genome-inserting genes used in transgenic insect-resistant crop development. Cry toxins are resistant to lepidopteran pests, such as Chilo suppressalis, a major insect pest of rice worldwide. Since Cry toxins exert their activity by binding to specific receptors in the midgut of target insects, identification of functional Cry toxin receptors in the midgut of C. suppressalis larvae is crucial to evaluate potential resistance mechanisms and develop effective strategies for inhibiting insect resistance. In this study, we isolated two aminopeptidase N genes (APN6... More

关键词

Aminopeptidase N; Bacillus thuringiensis; Chilo suppressalis; Cry toxin toxicity; RNA interference