In this study， we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought， using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling， root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves， respectively. Differences between the root and leaf proteome were most marked for photosynthesis， energy metabolism， and protein metabolism. In particu... More
In this study， we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought， using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling， root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves， respectively. Differences between the root and leaf proteome were most marked for photosynthesis， energy metabolism， and protein metabolism. In particular， some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought， in Brachypodium roots and leaves.
