Phosphagen kinases (PKs) are known to be distributed throughout the animal kingdom， but have recently been discovered in some protozoan and bacterial species. A recent search of the available bacterial genomes revealed 49 unique sequences that appear to code for an arginine kinase (AK). The distribution of sequences was highly skewed with thirty nine out the forty nine sequences being found in six Proteobacteria classes (α， β， δ， γ， ε， and ζ) which represented 46.6% of the 61，335 bacterial genomes available at JGI-IMG/M website. Moreover， twenty one of the unique and metagenome bAK sequences identified were from δ-Proteobacteria despite these representing only 0.88% of the total genomes a... More
Phosphagen kinases (PKs) are known to be distributed throughout the animal kingdom， but have recently been discovered in some protozoan and bacterial species. A recent search of the available bacterial genomes revealed 49 unique sequences that appear to code for an arginine kinase (AK). The distribution of sequences was highly skewed with thirty nine out the forty nine sequences being found in six Proteobacteria classes (α， β， δ， γ， ε， and ζ) which represented 46.6% of the 61，335 bacterial genomes available at JGI-IMG/M website. Moreover， twenty one of the unique and metagenome bAK sequences identified were from δ-Proteobacteria despite these representing only 0.88% of the total genomes available. Phylogenetic analyses revealed that the bacterial AK sequences were interpersed between basal species such as cnidarians， sponges and protozoa， displaying an unstable clustering that was dependent upon the parameters chosen for phylogenetic analysis. Three of these putative bacterial AK genes were cloned into the pET45 expression vector， expressed， and biochemically confirmed to be capable of phosphorylating arginine using ATP. Results of the kinetic analyses of the putative bAKs from Ahrensia， D. autotrophicum， and O. profundus show that the catalytic efficiencies with respect to arginine for each enzyme， measured at 10-10?M?s， fall within the range expected for competent arginine kinases.
