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The HIV-1 antisense protein ASP is a transmembrane protein of the cell surface and an integral protein of the HIV-1 viral envelope.

J. Virol.. 2019; 
AfframYvonne,ZapataJuan C,GholizadehZahra,TolbertWilliam D,ZhouWei,Iglesias-UsselMaria D,PazgierMarzena,RayKrishanu,LatinovicOlga S,RomerioF
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Monoclonal Antibody Services The hybridoma cell line expressing the anti-ASP 324.6 monoclonal antibody was generated at the Albert Einstein College of Medicine Hybridoma Core Facility on a fee-for-service basis using a synthetic 14-aa peptide (GenScript) with sequence identical to residues 97-110 of ASP from HIV-1NL4-3 (NH2-SLISPPPGLKISDP-COOH) and conjugated to keyhole limpet hemocyanin. Get A Quote

摘要

The negative strand of HIV-1 encodes a highly hydrophobic antisense protein (ASP) with no known homologs. The presence of humoral and cellular immune responses against ASP in HIV-1 patients indicate that ASP is expressed , but its role in HIV-1 replication remains unknown. We investigated ASP expression in multiple chronically infected myeloid and lymphoid cell lines using an anti-ASP monoclonal antibody (324.6) in combination with flow cytometry and microscopy approaches. At baseline and in the absence of stimuli, ASP shows a polarized sub-nuclear distribution, preferentially in areas with low content of suppressive epigenetic marks. However, following treatment with phorbol 12-myristate 13-acetate (PM... More

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