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Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7).

Cell. Mol. Life Sci.. 2019;

LiuZhiquan，ChenMao，ShanHuanhuan，ChenSiyu，XuYuxin，SongYuning，ZhangQuanjun，YuanHongming，OuyangHongsheng，LiZhanjun，LaiLian

Products/Services Used	Details	Operation
CRISPR Plasmids	Thus, we exploited this method to optimize the efciency of xBE4-Gam and xABE7.10, termed the enhanced xCas9-derived cytidine base editor (exBE4, bis-bpNLS BE4 with the Anc689 APOBEC, and GenScript codons) and adenine base editors (exABE, bis-bpNLS ABE7.10, and GenScript codons) (Fig. 4a, b).	Get A Quote

摘要

Evolved xCas9(3.7) variant with broad PAM compatibility has been reported in cell lines， while its editing efficiency was site-specific. Here， we show that xCas9(3.7) can recognize a broad PAMs including NGG， NGA， and NGT， in both embryos and Founder (F0) rabbits. Furthermore， the codon-optimized xCas9-derived base editors， exBE4 and exABE， can dramatically improve the base editing efficiencies in rabbit embryos. Our results demonstrated that the optimized xCas9 with expanded PAM compatibility and enhanced base editing efficiency could be used for precise gene modifications in organisms.