This study aimed to investigate the effects of winter and spring particulate matter (PM) on airway inflammation and allergies in a mouse asthma model. PM was collected during 7-28 February 2013 (winter) and during 7-28 April 2013 (spring) in Yonago， Japan. NC/Nga mice were co-sensitized using intranasal instillation of the PMs and Dermatophagoides farinae (Df) for 5 consecutive days， and were subsequently challenged using intranasal Df at 7 days after the last sensitization. At 24 h after the challenge， serum immunoglobulin levels， differential leukocyte counts， and inflammatory cytokines levels were measured in the mice's bronchoalveolar lavage fluid (BALF). Compared to co-sensitization using spring ... More
This study aimed to investigate the effects of winter and spring particulate matter (PM) on airway inflammation and allergies in a mouse asthma model. PM was collected during 7-28 February 2013 (winter) and during 7-28 April 2013 (spring) in Yonago， Japan. NC/Nga mice were co-sensitized using intranasal instillation of the PMs and Dermatophagoides farinae (Df) for 5 consecutive days， and were subsequently challenged using intranasal Df at 7 days after the last sensitization. At 24 h after the challenge， serum immunoglobulin levels， differential leukocyte counts， and inflammatory cytokines levels were measured in the mice's bronchoalveolar lavage fluid (BALF). Compared to co-sensitization using spring PM and Df， winter PM and Df induced greater increases in the BALF neutrophil and eosinophil counts and total serum IgE and IgG2a levels. Furthermore， winter PM-sensitized mice exhibited higher BALF levels of interleukin-5， interleukin-13， interleukin-6， and keratinocyte-derived chemokine. Therefore， we observed seasonal variations in the effects of PM on asthma-related airway inflammation. These findings suggest that the compositions of PM vary according to season， and that it is important to evaluate PM compositions in order to understand the associations between asthma and PM.
