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The C. difficile toxin B membrane translocation machinery is an evolutionarily conserved protein delivery apparatus.

Nat Commun. 2020; 
Orrell KE,, Mansfield MJ, Doxey AC, Melnyk RA,.
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Bacterial Expression System Full-length Sm protein (WP_073532240.1) lacking the first 35 amino acids was synthesized and codon-optimized for expression in E. coli (GenScript) and fused into a pET28a vector using In-Fusion HD cloning Get A Quote

摘要

Large Clostridial Toxins (LCTs) are a family of six homologous protein toxins that are implicated in severe disease. LCTs infiltrate host cells using a translocation domain (LCT-T) that contains both cell-surface receptor binding sites and a membrane translocation apparatus. Despite much effort, LCT translocation remains poorly understood. Here we report the identification of 1104 LCT-T homologs, with 769 proteins from bacteria outside of clostridia. Sequences are widely distributed in pathogenic and host-associated species, in a variety of contexts and architectures. Consistent with these homologs being functional toxins, we show that a distant LCT-T homolog from Serratia marcescens acts as a pH-dependent tra... More

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