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Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.

Nucleic Acids Res. 2020; 
Li N,,, Wang J, Wallace SS, Chen J, Zhou J, D'Andrea AD,,.
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ORF cDNA Clones/MolecularCloud The EGFP-FANCD2 and NEIL3-HA plasmids were described previously (20,39). The RUVBL1-Flag (#OHu26261) and RUVBL2-Flag (#OHu25367) plasmids were purchased from GenScript. Get A Quote

摘要

The NEIL3 DNA glycosylase is a base excision repair enzyme that excises bulky base lesions from DNA. Although NEIL3 has been shown to unhook interstrand crosslinks (ICL) in Xenopus extracts, how NEIL3 participants in ICL repair in human cells and its corporation with the canonical Fanconi anemia (FA)/BRCA pathway remain unclear. Here we show that the NEIL3 and the FA/BRCA pathways are non-epistatic in psoralen-ICL repair. The NEIL3 pathway is the major pathway for repairing psoralen-ICL, and the FA/BRCA pathway is only activated when NEIL3 is not present. Mechanistically, NEIL3 is recruited to psoralen-ICL in a rapid, PARP-dependent manner. Importantly, the NEIL3 pathway repairs psoralen-ICLs without generating... More

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