至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Strain-Dependent RstA Regulation of Clostridioides difficile Toxin Production and Sporulation

J Bacteriol. 2020-01; 
Edwards AN, Krall EG, McBride SM.
Products/Services Used Details Operation
DNA Sequencing Sequencing of the C. difficile tcdE open reading frames was performed by GenScript (Piscataway, NJ). Get A Quote

摘要

The anaerobic spore former Clostridioides difficile causes significant diarrheal disease in humans and other mammals. Infection begins with the ingestion of dormant spores, which subsequently germinate within the host gastrointestinal tract. There, the vegetative cells proliferate and secrete two exotoxins, TcdA and TcdB, which cause disease symptoms. Although spore formation and toxin production are critical for C. difficile pathogenesis, the regulatory links between these two physiological processes are not well understood and are strain dependent. Previously, we identified a conserved C. difficile regulator, RstA, that promotes sporulation initiation through an unknown mechanism and directly and indirectly r... More

关键词

Clostridioides difficile ; Clostridium difficile ; CodY; RstA; TcdA; TcdR; anaerobe; gene regulation; spore; sporulation; toxin production; transcriptional regulation