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Evaluation of Leishmania infantum pyridoxal kinase protein for the diagnosis of human and canine visceral leishmaniasis

Immunol Lett. 2020; 
Oliveira-da-Silva JA, Machado AS, Ramos FF, Tavares GSV, Lage DP, Ludolf F, Steiner BT, Reis TAR, Santos TTO, Costa LE, Martins VT, Galvani NC, Chaves AT, Oliveira JS, Chávez-Fumagalli MA, de Magalhães-Soares DF, Duarte MC, Menezes-Souza D, Silveira JAG, Moreira RLF, Machado-de-Ávila RA, Tupinambás U, Gonçalves DU, Coelho EAF.
Products/Services Used Details Operation
PCR Cloning and Subcloning … 23 Cloning, expression, and purification of the recombinant proteins The L infantum gene encoding for the PK (XP_0014669751) was commercially synthetized into pET-28a(+)-TEV vector, by using the BamHI and EcoRI enzymes (Genscript®, USA) … Get A Quote

摘要

Visceral leishmaniasis (VL) is a highly neglected disease that is present in several countries worldwide. Present-day treatments against this disease are unsuitable, mainly due to the toxicity and/or high cost of drugs. In addition, the development of vaccines is still insufficient. In this scenario, a prompt VL diagnosis was deemed necessary, although sensitivity and/or specificity values of the tests have been. In this context, new antigenic candidates should be identified to be employed in a more precise diagnosis of canine and human VL. In this light, the present study evaluated the diagnostic efficacy of the Leishmania infantum pyridoxal kinase (PK) protein, applied in its recombinant version (rPK). In add... More

关键词

Diagnosis; Leishmania infantum; Pyridoxal kinase; Recombinant proteins; Synthetic peptides; Visceral leishmaniasis