| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | … manufacturer's instructions 23 Construction of an expression vector with a single-chain Fv fragment The DNA fragment coding scFv VL-linker-VH of anti-CD22 gene (Fig 1) was synthesized (GenScript USA Inc, US) VL contains … | Get A Quote |
Single-chain variable fragment (scFv) antibodies are fusion proteins of the variable regions of the heavy and light chains of immunoglobulins connected with a short linker peptide. They possess unique and superior features compared to whole antibodies for immunotherapy of various carcinomas, including hematologic B-cell malignancies. In the presented study we obtained efficient production of the recombinant anti-CD22 scFv in Escherichia coli expression system. The active recombinant protein was successfully recovered from inclusion bodies. Assays were performed to assess the in vitro targeting properties and specificity of the obtained anti-CD22 scFv antibody in the CD22 positive and negative lymphoma cell line... More
