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Protective effect of microRNA‑381 against inflammatory damage of endothelial cells during coronary heart disease by targeting CXCR4

Mol Med Rep. 2020; 
Li Y, Huang J, Yan H, Li X, Ding C, Wang Q, Lu Z.
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Mutagenesis Services … dual‑luciferase reporter vectors (Promega corporation) Mutations in the mir‑381 target site of the 3'‑uTr of cXcr4 were generated using a Quickchange Site‑directed Mutagenesis kit (genScript) 293 cells (american Type culture … Get A Quote

摘要

Coronary heart disease (CHD) is the leading cause of human morbidity and mortality worldwide. MicroRNA (miRNA) profiling is an innovative method of identifying biomarkers for many diseases and may be a powerful tool in the diagnosis and treatment of CHD. The present study aimed to analyze the effects of miRNA (miR)‑381 on the inflammatory damage of endothelial cells during CHD. A total of 21 patients with CHD and 21 healthy control patients were enrolled in this study. Reverse transcription‑quantitative PCR, western blotting and immunofluorescence assays were conducted to examine the expression levels of miR‑381, C‑X‑C chemokine receptor type 4 (CXCR4), Bcl‑2, Bax, Cleaved‑Caspases‑3 and ... More

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