Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | … mouse-derived anti-FLAG monoclonal antibodies conjugated to horseradish peroxidase (HRP; 337 1:5,000; GenScript, Piscataway, NJ) Chemiluminescence signals were detected using a 338 ChemiDoc MP imaging system (Bio-Rad, Hercules, CA) … | Get A Quote |
The rise of Gram-negative pathogens expressing metallo-β-lactamases (MBLs) is a growing concern, threatening the efficacy of β-lactam antibiotics, in particular, the carbapenems. There are no inhibitors of MBLs in current clinical use. Aspergillomarasmine A (AMA) is an MBL inhibitor isolated from Aspergillus versicolor with both in vitro and in vivo ability to rescue meropenem activity in MBL-producing bacteria. Here we systematically explored the pairing of AMA with six β-lactam antibiotic partners against nineteen MBLs from each subclass (B1, B2, B3). Cell-based assays with Escherichia coli and Klebsiella pneumoniae showed that bacteria producing NDM-1 and VIM-2 of subclass B1 were the most susceptible to ... More