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A genome-wide CRISPR-Cas9 screen identifies the dolichol-phosphate mannose synthase complex as a host dependency factor for dengue virus infection

J Virol. 2020; 
Labeau A, Simon-Loriere E, Hafirassou ML, Bonnet-Madin L, Tessier S, Zamborlini A,, Dupré T, Seta N, Schwartz O, Chaix ML,, Delaugerre C,, Amara A, Meertens L.
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CRISPR Libraries … 382 383 Pooled CRIPSR screen and plasmid constructs The GeCKO v2 human CRISPR 384 pooled libraries (A and B) encompassing 123,411 different sgRNA targeting 19,050 385 genes were purchased from GenScript Genome-wide CRISPR-Cas9-edited HAP1 386 Get A Quote

摘要

Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available. Like other viruses, DENV relies heavily on the host cellular machinery for productive infection. Here, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection. We identified DPM1 and 3, two subunits of the ER resident DPM synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses such as Zika virus (ZIKV). DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM) which serves as mannosyl donor in pathways leading to N-glycosylation, GPI ancho... More

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