| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | Porcine peripheral blood mononuclear cell (PBMC) were prepared from fresh GenScript, Nanjing, Jiangsu, China) at the concentration of 40 ng/mL for 6 consecutive days, and only the suspended cells (with typical dendritic cells morphology) were collected.... The binding of antibody to the corresponding antigen was detected using horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibodies (GenScript) and visualized by a TMB substrate kit (Tiangen Biotech, Beijing, China). | Get A Quote |
Porcine reproductive and respiratory syndrome (PRRS) is one of the most common diseases in the global swine industry. PRRSV infection is highly restricted to cells of the monocyte-macrophage lineage. However, the lack of antibodies to swine monocyte-macrophage lineage markers significantly hampers PRRSV research. In this study, we have developed a monoclonal antibody against the swine leukocyte antigen (SLA)-DRα chain and confirmed its reactivity with endogenous expressed SLA-DR in a variety of cell lines and primary swine antigen-presenting cells (PAMs, PBMC and BM-DCs). Moreover, the level of SLA-DR expression after PRRSV infection were evaluated by our homemade Mab and a commercial anti-SLA-DR antibody. Bas... More
