| Products/Services Used | Details | Operation |
|---|---|---|
| Custom DNA/RNA Oligos | 011 Continued REAGENT or RESOURCE Oligonucleotides gRNA for CSE KO (exon 5) 5’-GGAGACATTATTTTGGTCGTGG-3’ gRNA for CSE KO (exon 1) 5’-TTCCAACATTTGGCCACGCAGG-3’ gRNA for SQR KO (exon 8) 5’-GTCCTCAAGACCAGTCCTGTGG-3’ gRNA for SQR KO (exon 3) 5’-TGCCGTGGGACGACCAGATG-3’ gRNA for MPST KO (exon 3) 5’-CGCGTTACCGTCTCGGGGCT-3’ Recombinant DNA Plasmid: pLPCX cyto-roGFP2 Plasmid: pLPCX mito-roGFP2 Plasmid: pLPCX cyto-roGFP2-Orp1 Plasmid: pLPCX mito-roGFP2-Orp1 Software and Algorithms MARS data analysis software OriginPro 2017G SOURCE IDENTIFIER Sigma-Aldrich Target ID HS0000181212 Sigma-Aldrich Target ID HS0000181204 Sigma-Aldrich Target ID HS0000225831 Genscript Genscript Gutscher et al.... Generation of CRISPR/Cas9-Mediated Knockout Cells The following guide RNAs (gRNAs) were used in the study: for CSE: exon 5 (5’-GGAGACATTATTTTGGTCGTGG-3’) and exon 1 (5’-TTCCAACATTTGGCCACGCAGG-3’) (purchased from Sigma-Aldrich); for SQR: exon 8 (5’-GTCCTCAAGACCAGTCCTGTGG-3’) (Sigma-Aldrich) and exon 3 (5’-TGCCGTGGGACGACCAGATG-3’) (Genscript); for MST: exon 4 (5’-CGCGTTACCGTCTCG GGGCT-3’) (Genscript). | Get A Quote |
The cysteine prodrug N-acetyl cysteine (NAC) is widely used as a pharmacological antioxidant and cytoprotectant. It has been reported to lower endogenous oxidant levels and to protect cells against a wide range of pro-oxidative insults. As NAC itself is a poor scavenger of oxidants, the molecular mechanisms behind the antioxidative effects of NAC have remained uncertain. Here we show that NAC-derived cysteine is desulfurated to generate hydrogen sulfide, which in turn is oxidized to sulfane sulfur species, predominantly within mitochondria. We provide evidence suggesting the possibility that sulfane sulfur species produced by 3-mercaptopyruvate sulfurtransferase and sulfide:quinone oxidoreductase are the actual... More
