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Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization.

Sci Rep. 2016;

Zhang G, Yang XX, Leung PM, He LS, Chan TY, Yan GY, Zhang Y, Sun J, Xu Y, Qian PY.

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	Briefly, full-length SIPC was cloned into a pFastBacTMHT A vector (Life Technology, Grand Island, NY, USA) at both BamH I and Hind III sites using a CloneEZ PCR Cloning kit (Genscript, Nanjing, China) and transformed into Top10 competent E.... The proteins in each fraction were separated in a 4–20% gradient SDS-PAGE gel (Genscript, Nanjing, China) and stained using Coomassie blue G250.	Get A Quote

摘要

Settlement-inducing protein complex (SIPC) is a pheromone that triggers conspecific larval settlement in the barnacle Amphibalanus amphitrite. In the present study, immunostaining and scanning electron microscopy of SIPC revealed signals in the frontal horn pores and the secretions from carapace pores, suggesting that SIPC might be directly secreted from these organs in A. amphitrite cyprids. Further observations showed that the frontal horn pores could contact surfaces while cyprids were "walking". Immunostaining for SIPC on the contacted surfaces displayed SIPC signals. These signals were similar to the frontal horn pores in size and morphology, suggesting that frontal horn pores might deposit SIPC. Besides, ... More