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Development of immunochromatographic colloidal gold test strip for rapid detection of Haemophilus influenzae in clinical specimens.

J Appl Microbiol. 2017; 
Kong MM, Yang B, Gong CJ, Wang H, Li X, Zhao KS, Li JJ, Wu F, Liu X, Hu Z.
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Recombinant Antibody Services To facilitate the construction of the recombinant plasmid, the restriction site NdeI and XhoI were designed on the flanks of the gene encoding for the deduced antigen, and the gene was synthesized (Nanjing Genscript Co. Get A Quote

摘要

A simple and fast test strip for simultaneous detection of Haemophilus influenzae, based on the theory of colloidal gold immunochromatography was developed.,In this study, monodispersional colloidal gold and gold-labelled polyclonal antibody (AbP6Line) specific to the linear antigenic determinant OMP6 of H. influenzae were prepared, and identified by UV-visible spectroscopy (200-700 nm) and transmission electron microscopy. The test strip was assembled on a plastic backing containing a sample pad, a conjugate pad, an absorbent pad and a nitrocellulose membrane, on which test and control lines were stuck. The strip showed us specific recognization of H. influenzae and did not identify other common respiratory p... More

关键词

Haemophilus influenzae ; colloidal gold; immunochromatography; rapid detection; test strip