| Products/Services Used | Details | Operation |
|---|---|---|
| Cellular Analysis | Endotoxins levels were evaluated by using the ToxinSensorTM Chromogenic Limulus Amoebocyte Lysate assay kit (GenScript, USA), in accordance with the manufac- turer’s instructions. | Get A Quote |
Minicircle DNA is a new biotechnological product with beneficial therapeutic perspectives for gene therapy because it is constituted only by the eukaryotic transcription unit. These features improve minicircle DNA safety and increase its therapeutic effect. However, being a recently developed product, there is a need to establish efficient purification methodologies, enabling the recovery of the supercoiled minicircle DNA isoform. Thus, this work describes the minicircle DNA purification using an anion exchange monolithic support. The results show that with this column it is possible to achieve a good selectivity, which allows the isolation of the supercoiled minicircle DNA isoform from impurities. Overall, thi... More
