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TcpM: a novel relaxase that mediates transfer of large conjugative plasmids from Clostridium perfringens.

Mol Microbiol. 2016; 
Wisniewski JA,, Traore DA,, Bannam TL,, Lyras D,, Whisstock JC,, Rood JI,.
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摘要

Conjugative transfer of toxin and antibiotic resistance plasmids in Clostridium perfringens is mediated by the tcp conjugation locus. Surprisingly, neither a relaxase gene nor an origin of transfer (oriT) has been identified on these plasmids, which are typified by the 47 kb tetracycline resistance plasmid pCW3. The tcpM gene (previously called intP) encodes a potential tyrosine recombinase that was postulated to be an atypical relaxase. Mutagenesis and complementation studies showed that TcpM was required for wild-type transfer of pCW3 and that a tyrosine residue, Y259, was essential for TcpM activity, which was consistent with the need for a relaxase-mediated hydrophilic attack at the oriT site. Other catal... More

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