| Products/Services Used | Details | Operation |
|---|---|---|
| Mutagenesis Services | pcD1ME variants containing other amino acid substitutions at E87 or E107 that were used for the experiments shown in Figure 2D were constructed using pcD1ME as a template and a site-directed mutagenesis service provided by GenScript.... Other pcDNA3-based plasmids expressing the prM and E of the non-prototype DENV-1 to DENV-4 strains (pcBDV1 to pcBDV4 used for the experiments shown in Figure 3) were constructed using DNA fragments corresponding to the prM/E region synthesized by GenScript based on nucleotide sequence information available in GenBank. | Get A Quote |
Dengue is a globally important disease caused by four serotypes of dengue virus. Dengue vaccine development has been hampered by antigenic cross-reactivity among serotypes, which potentially causes antibody-dependent enhancement of infection and disease severity. Here we found that a single amino acid substitution in the envelope protein at position 87 from aspartic acid to asparagine or at position 107 from leucine to phenylalanine is critical for suppressing the induction of infection-enhancing antibody in a mouse model. The site and type of amino acid substitution were determined via neutralization escape using an enhancing-activity-only monoclonal antibody that was engineered to reveal neutralizing activity... More
