| Products/Services Used | Details | Operation |
|---|---|---|
| ORF cDNA Clones/MolecularCloud | The sequences of the peptide library, excluding the kinase domain of HER2/neu and consisting of 7 or 8 overlapping peptides, were designed using the peptide library design and calculator tool from Sigma-Aldrich and ordered from GenScript.... Cox-2 DNA was amplified using a house mouse Ptgs2 cDNA ORF clone (oMu19313, GenScript) and following primers: mouse Cox-2 forward (KpnI); 5’- GAG CTC GGT ACC GCC ACC ATG CTC TTC-3’, mouse Cox-2 reverse (NotI); 5’-GAG GCT GAT GCG GCC GCT TAT CAC TTA TCG TC-3’. | Get A Quote |
GM-CSF as an adjuvant has been shown to promote antitumor immunity in mice and humans; however, the underlying mechanism of GM-CSF-induced antitumor immunity remains incompletely understood. In this study, we demonstrate that GM-CSF potentiates the efficacy of cancer vaccines through IL9-producing Th (Th9) cells. GM-CSF selectively enhanced Th9 cell differentiation by regulating the COX2-PGE2 pathway while inhibiting the differentiation of induced regulatory T (iTreg) cells in vitro and in vivo GM-CSF-activated monocyte-derived dendritic cells converted tumor-specific naïve Th cells into Th9 cells, and delayed tumor growth by inducing antitumor CTLs in an IL9-dependent manner. Our findings reveal a mechanism f... More
