Products/Services Used | Details | Operation |
---|---|---|
Monoclonal Antibody Services> | The following primar y antibodies were used for this study : anti-PARP6 (rabbit polyclonal, Sigma Prestige® HPA026991, 1:1000); anti-GAPDH (mouse monoclonal, Santa Cruz Biotechnology clone 6C5, 1:5000); anti-β -actin (mouse monoclonal, GenScript THETM clone 2D1D10, 1:1000); anti-HA (mouse mono- clonal, Covance clone 16B12, 1:1000); anti-GFP (chicken polyclonal, Abcam ab13970, 1:5000); HRP-conjugated streptavidin (Jackson ImmunoResearch, 0. | Get A Quote |
Mono-ADP-ribosylation (MARylation) of mammalian proteins was first described as a post-translational modification catalyzed by bacterial toxins. It is now known that endogenous MARylation occurs in mammalian cells and is catalyzed by 11 members of the poly-ADP-ribose polymerase (PARP) family of proteins (17 in humans). The physiological roles of these PARPs remain largely unknown. Here we demonstrate that PARP6, a neuronally enriched PARP that catalyzes MARylation, regulates hippocampal dendrite morphogenesis, a process that is critical for proper neural circuit formation during development. Knockdown of PARP6 significantly decreased dendritic complexity in embryonic rat hippocampal neurons in culture and in vi... More