至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Redox sensitivity of the MyD88 immune signaling adapter.

Free Radic Biol Med. 2016; 
Stottmeier B, Dick TP.
Products/Services Used Details Operation
Mutagenesis Services Mutations were introduced using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) or by insertion of synthetic, mutated ORF fragments (GenScript) using available restriction sites. Get A Quote

摘要

The transcription factor nuclear factor-κB (NF-κB) mediates expression of key genes involved in innate immunity and inflammation. NF-κB activation has been repeatedly reported to be modulated by hydrogen peroxide (H2O2). Here, we show that the NF-κB-activating signaling adapter myeloid differentiation primary response gene 88 (MyD88) is highly sensitive to oxidation by H2O2 and may be redox-regulated in its function, thus facilitating an influence of H2O2 on the NF-κB signaling pathway. Upon oxidation, MyD88 forms distinct disulfide-linked conjugates which are reduced by the MyD88-interacting oxidoreductase nucleoredoxin (Nrx). MyD88 cysteine residues functionally modulate MyD88-dependent NF-κB activation... More

关键词

Hydrogen peroxide; MyD88; NF-κB; Redox signaling; Thiol oxidation