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Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice.

Biotechnol Rep (Amst). 2018; 
Ruiz V,, Baztarrica J, Rybicki EP, Meyers AE, Wigdorovitz A,.
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud benthamiana and synthesized as a single ORF by GenScript (Piscataway, NJ, USA). Get A Quote

摘要

Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3-4 mg/kg of fresh leaf tissue. Both groups of mice immunized wi... More

关键词

Foot-and-mouth disease virus; Nicotiana benthamiana; Transient expression; Virus-like particles