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ORF cDNA Clones/MolecularCloud> | The M lp124478 cod ing sequence w ithout the s igna l pept ide ( lack ing am ino ac ids 1–27 , 1 0 hereafter referred to as Mlp124478) was ordered from GenScript in pUC57 in lyophilized form, and primer pairs (Supplementary Table 1, Primers Nos 1–3) were used to amplify the open reading frame (ORF) of Mlp124478 from pUC57 by the polymerase chain reaction (PCR). | Get A Quote |
The basidiomycete Melampsora larici-populina causes poplar rust disease by invading leaf tissues and secreting effector proteins through specialized infection structures known as haustoria. The mechanisms by which rust effectors promote pathogen virulence are poorly understood. The present study characterized Mlp124478, a candidate effector of M. larici-populina. We used the models Arabidopsis thaliana and Nicotiana benthamiana to investigate the function of Mlp124478 in plant cells. We established that Mlp124478 accumulates in the nucleus and nucleolus, however its nucleolar accumulation is not required to promote growth of the oomycete pathogen Hyaloperonospora arabidopsidis. Stable constitutive expression of... More