| Products/Services Used | Details | Operation |
|---|---|---|
| Mutagenesis Services | A R24R63FurA_pET-28a plasmid containing a double mutation in two arginines located in positions 24 and 73 and a R70FurA_pET-28a plasmid holding a single mutation in arginine located in position 70 were obtained by site-directed mutagenesis (synthesized by GenScript, Piscataway, NJ, USA). | Get A Quote |
2-oxoglutarate (2-OG) is a central metabolite that acts as a signaling molecule informing about the status of the carbon/nitrogen balance of the cell. In recent years, some transcriptional regulators and even two-component systems have been described as 2-OG sensors. In the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, two master regulators, NtcA and FurA, are deeply involved in the regulation of nitrogen metabolism. Both of them show a complex intertwined regulatory circuit to achieve a suitable regulation of nitrogen fixation. In this work, 2-OG is found to bind FurA, modulating the specific binding of FurA to the ntcA promoter. This study provides evidence of a new additional control point in the com... More
