至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

FliC's Hypervariable D3 Domain Is Required for Robust Anti-Flagellin Primary Antibody Responses.

Immunohorizons. 2019; 
López-Yglesias AH, Lu CC, Zhao X, Chou T, VandenBos T, Strong RK, Smith KD.
Products/Services Used Details Operation
Bacterial Expression System DNA encoded FliCDNaip5/6, FliCDTLR5, FliCDTLR5/Naip5/6, FliCDD3, and FliCDD0/D1, (GenScript, Piscataway, NJ) and transformed into flagellin negative BL21 Escherichia coli cells for expression (Invitrogen, Life Technologies, Thermo Fisher Scientific, Grand Island, NY). Get A Quote

摘要

Bacterial flagellin is a well-known agonist of the innate immune system that induces proinflammatory responses through the TLR5 and Naip5/6 recognition pathways. Several clinical trials investigating flagellin fusion proteins have demonstrated promising results for inducing protective immunity toward influenza virus, which has been largely attributed to flagellin's ability to activate TLR5. Our laboratory previously demonstrated that the Salmonella enterica serovar Typhimurium flagellin protein, FliC, induces Ab responses in mice through a third pathway that is independent of TLR5, Casp1/11, and MyD88. In this study, we further define the structural features of FliC that contribute to this unknown third pathway... More

关键词