| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | SssI) New England Biolabs M0226S M-MLV Reverse Transcriptase Promega M170A Taq DNA Polymerase GenScript E00007 FastDigest KpnI Fermentas FD0524 FastDigest HindIII Fermentas FD0505 FastDigest XhoI Fermentas FD0694 X-Gal Sigma-Aldrich 3117073001 Corning 10% SDS Corning MT-46040-CI Yeast tRNA ThermoFisher AM7119 Corning â 500 mL RPMI 1640 Corning 10-040-CV HEPES Buffer Corningâ 100 mL Penicillin-Streptomycin Solution, 100x Sodium Pyruvate (100 mM) Corning MT-25060-CI Corning 30-002-CI GIBCO 11360070 MEM Non-Essential Amino Acids Solution (100X) HyClone SH3023801 Critical Commercial Assays ImmPRESS HRP Universal Antibody (Anti-Mouse IgG/Anti-Rabbit IgG) Vector Laboratories MP-7500 Mini-PROTEAN â TGX Gels Bio-Rad 4561096 Power SYBR Green PCR Master Mix ThermoFisher 4367659 Chromatin Immunoprecipitation (ChIP) Assay Kit Millipore 17295 TA Cloning Kit, with pCR2. | Get A Quote |
IL-10 functions as a suppressor of colitis and colitis-associated colon cancer, but it is also a risk locus associated with ulcerative colitis. The mechanism underlying the contrasting roles of IL-10 in inflammation and colon cancer is unknown. We report here that inflammation induces the accumulation of CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) that express high levels of IL-10 in colon tissue. IL-10 induces the activation of STAT3 that directly binds to the Dnmt1 and Dnmt3b promoters to activate their expression, resulting in DNA hypermethylation at the Irf8 promoter to silence IRF8 expression in colon epithelial cells. Mice with Irf8 deleted in colonic epithelial cells exhibit significantly higher ... More
