至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

N-glycosylation of the protein disulfide isomerase Pdi1 ensures full Ustilago maydis virulence.

PLoS Pathog. 2019; 
Marín-Menguiano M, Moreno-Sánchez I, Barrales RR, Fernández-Álvarez A, Ibeas JI.
Products/Services Used Details Operation
Mutagenesis Services 0 tool were replaced by site-direct mutagenesis using GenScript (Piscataway, USA), generating the plasmid pUC57-pdi1mutO-gly, with Sma1 and NotI restriction sites. Get A Quote

摘要

Fungal pathogenesis depends on accurate secretion and location of virulence factors which drive host colonization. Protein glycosylation is a common posttranslational modification of cell wall components and other secreted factors, typically required for correct protein localization, secretion and function. Thus, the absence of glycosylation is associated with animal and plant pathogen avirulence. While the relevance of protein glycosylation for pathogenesis has been well established, the main glycoproteins responsible for the loss of virulence observed in glycosylation-defective fungi have not been identified. Here, we devise a proteomics approach to identify such proteins and use it to demonstrate a role for ... More

关键词