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The FeoC [4Fe-4S] Cluster Is Redox-Active and Rapidly Oxygen-Sensitive.

Biochemistry. 2019; 
Smith AT, Linkous RO, Max NJ, Sestok AE, Szalai VA, Chacón KN.
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Bacterial Expression System DNA encoding for the genes corresponding to FeoC from Escherichia coli (strain K-12) (Uniprot identifier P64638) (EcFeoC) and from Klebsiella pneumoniae (strain 342) (Uniprot identifier B5XTS6) (KpFeoC) were commercially synthesized by GenScript (Piscataway, NJ), with additionally engineered DNA sequences encoding for a C-terminal TEV-protease cleavage site (ENLYFQG) or with an additionally engineered DNA sequence encoding for an N-terminal maltose-binding protein sequence (based on P0AEX9: Escherichia coli (K-12) malE gene product) followed by a Tobacco Etch Virus (TEV)-protease cleavage site. Get A Quote

摘要

The acquisition of iron is essential to establishing virulence among most pathogens. Under acidic and/or anaerobic conditions, most bacteria utilize the widely distributed ferrous iron (Fe2+) uptake (Feo) system to import metabolically-required iron. The Feo system is inadequately understood at the atomic, molecular, and mechanistic levels, but we do know it is composed of a main membrane component (FeoB) essential for iron translocation, as well as two small, cytosolic proteins (FeoA and FeoC) hypothesized to function as accessories to this process. FeoC has many hypothetical functions, including that of an iron-responsive transcriptional regulator. Here, we demonstrate for the first time that Escherichia coli... More

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