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Quantitative and Label-Free Detection of Protein Kinase A Activity Based on Surface-Enhanced Raman Spectroscopy with Gold Nanostars.

Anal Chem. 2018; 
He S, Kyaw YME, Tan EKM, Bekale L, Kang MWC, Kim SS, Tan I, Lam KP, Kah JCY,.
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Biochemicals … Bioconjugation of Kem to BSA. Kem powder with 95% purity [GenScript (Hong Kong) Limited] was conjugated to BSA by carbodiimide cross-linker chemistry.(26) Briefly, BSA was dissolved in phosphate buffer at pH 5 to facilitate efficient cross-linking … Get A Quote

摘要

The activity of extracellular protein kinase A (PKA) is known to be a biomarker for cancer. However, conventional PKA assays based on colorimetric, radioactive, and fluorometric techniques suffer from intensive labeling-related preparations, background interference, photobleaching, and safety concerns. While surface-enhanced Raman spectroscopy (SERS)-based assays have been developed for various enzymes to address these limitations, their use in probing PKA activity is limited due to subtle changes in the Raman spectrum with phosphorylation. Here, we developed a robust colloidal SERS-based scheme for label-free quantitative measurement of PKA activity using gold nanostars (AuNS) as a SERS substrate functionalize... More

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