至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

DNA methylation pattern of the goat PITX1 gene and its effects on milk performance.

Arch Anim Breed. 2019; 
Zhao H, Zhang S, Wu X, Pan C, Li X, Lei C, Chen H, Lan X.
Products/Services Used Details Operation
DNA Sequencing … Purified fragments were cloned into the pGEM-T Easy Vector (Promega, WI, USA) system. The colony PCR was used to identify the positive inserts, which were sequenced via sequencing service (GenScript, Nanjing, China) … Get A Quote

摘要

Paired-like homeodomain transcription factor 1 (PITX1) is a pivotal gene in the hypothalamic-pituitary-adrenal axis, which is a well-known pathway affecting lactation performance. The aim of this study was to analyze the DNA methylation profile of the PITX1 gene and its relevance to milk performance in Xinong Saanen dairy goats; thus, potential epigenetic markers of lactation performance were identified. A total of 267 goat blood samples were divided into "low" and "high" groups according to two milk traits: the average milk yield (AMY) and the average milk density (AMD). One CpG island in the 3 ' -flanking region of the PITX1 gene was identified as being related to milk performance. Fisher's exact test demon... More

关键词