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Nucleic Acid Purification & Analysis> | … All protein concentration was quantified by BCA method. 25μg of proteins from each group were loaded on ExpressPLUSTMPAGE gels (GenScript, USA) and then transferred on PVDF membranes before incubated with primary antibodies (1:2000) overnight … | Get A Quote |
Nanosecond pulsed electric fields (nsPEFs) is emerged as a potential curative modality to ablate hepatocellular carcinoma (HCC). The application of local ablation is usually limited by insufficiency of liver function. While baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi, has been proven to possess both anti-tumor and protective effects. Our study aimed to estimate different responses of hepatic cancer cells and hepatocytes to the combination of nsPEFs and baicalin. Cell viability, apoptosis and necrosis, mitochondrial transmembrane potential (MTP) and reactive oxygen species (ROS) were examined by CCK-8, FCM, JC-1 and fluorescent probe, respectively. After treatment by nsPEFs, most hepatocyt... More