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Dynamic Magnetic Nanomixers for Improved Microarray Assays by Eliminating Diffusion Limitation.

Adv Healthc Mater. 2019; 
Xiong Q, Lim AE, Lim Y, Lam YC, Duan H.
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Bacterial Expression System … E. coli O157:H7 ssDNA and fluorescence labeled DNA probe were order from Sangon Biotechnology. PSA standard protein was purchased from Fitzgerald. Capture and detection antibody to PSA were obtained from GenScript Get A Quote

摘要

Microarrays are widely used in high-throughput analysis of DNA, protein, and small molecules. However, the majority of microarray assays need improved assay speed and sensitivity due to the slow molecular diffusion from bulk solutions to probe surfaces. Here, a new class of magnetic nanomixers in DNA and protein microarray assays is reported to eliminate the diffusion constraint through dynamic mixing. It is demonstrated that the dynamic nanomixers can improve the assay kinetics at least by a factor of 4 and 2 for DNA and protein microarray assays, respectively. By using the dynamic nanomixers, the sensitivities of detecting Escherichia coli O157:H7 DNA and prostate specific antigen increase by more than four-f... More

关键词

diffusion limitation; dynamic nanomixers; magnetic nanochains; microarrays