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Molecular evidence for the adaptive evolution of Geobacter sulfurreducens to perform dissimilatory iron reduction in natural environments.

Mol Microbiol. 2019; 
Liu X, Ye Y, Xiao K, Rensing C, Zhou S.
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Nucleic Acid Purification & Analysis … Equal amounts of protein were loaded on 12.5% Tris-tricine polyacrylamide gels (Genscript, Nanjing, China) and separated by SDS-PAGE. After … Columns (Qiagen, Hilden, Germany), the protein antigen was used for the immunization of rabbits (GenScript, Nanjing, China) … Get A Quote

摘要

The electrically conductive pili (e-pili) of Geobacter species enable extracellular electron transfer to insoluble metallic minerals, electrodes and other microbial species, which confers biogeochemical significance and global prevalence on Geobacter in diverse anaerobic environments. E-pili are constructed by truncated PilA which is considered to have evolved from full-length pilin by gene fission under positive evolutionary selection. However, this hypothesis is based on phylogenetic analysis and has not yet been experimentally confirmed. Here, we reconstructed an ancestral strain of G. sulfurreducens (designated COMB) carrying full-length PilA by combining genes GSU1496 and GSU1497. The results demonstrated ... More

关键词

Geobacter sulfurreducens ; adaptive evolution; dissimilatory iron reduction; e-pili; truncated pilin