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Fluorometric determination of microRNA using arched probe-mediated isothermal exponential amplification combined with DNA-templated silver nanoclusters.

Mikrochim Acta. 2019; 
Wu H, Wu J, Liu Y, Wang H, Zou P.
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Custom DNA/RNA Oligos … Materials and reagents. The HPLC-purified DNA oligonucleotides (S1, S2, S3, S4, S5 and S6) and HPLC-purified microRNAs (microRNA-141, microRNA-200a, microRNA-200b, microRNA-429 and microRNA-199a) were synthesized by Genscript (Nanjing) Co., Ltd … Get A Quote
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摘要

A highly sensitive fluorometric method is described for the determination of microRNA-141. It is based on the use of arched probe-mediated isothermal exponential amplification reaction (EXPAR) and of DNA-templated silver nanoclusters (DNA-AgNCs). The EXPAR utilizes microRNA-141 as the trigger, polymerases and endonucleases as amplification activators, and two arched probes as exponential amplification templates. This enables the conversion of microRNA to a large number of reporter sequences under isothermal conditions within minutes. The generated reporter sequences act as scaffolds for the synthesis of fluorescent DNA-AgNCs by reduction of Ag (I) with NaBH4. The DNA-AgNCs function as signalling fluorophores wi... More

关键词

Arched probe; DNA polymerase; Human serum; Isothermal amplification; MicroRNA-141; Nicking endonuclease; Silver nanoclusters