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Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator.

Cells. 2019; 
Lu Y,, Dai J,,, Kong N, Liu J, Gong J, Yao Y.
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Nucleic Acid Purification & Analysis … 2.5. SDS-PAGE and Silver Staining. For each sample, 20 µL protein extractions were loaded on 4%–20% ExpressPlus™ PAGE Gel (Genscript, Nanjing, China) followed by electrophoresis. Silver staining used a rapid silver staining kit (Beyotimes, Shanghai, China) for detection … Get A Quote

摘要

The fabrication of shape-controlled nanocarriers is critical for efficient delivery of biomolecules across the cell membrane. Surface coating of the nanocarrier can improve internalization efficiency. Here, we developed a facile method of silicon nanorod fabrication leading to a controlled size and shape. We then systematically evaluated five surface modifications with membrane proteins from different cancer cell lines including MCF7, MD231, Hela, Panc-PDX, and Panc-1. We demonstrated that silicon nanorods coated with either a homolytic or heterolytic membrane protein coating have significantly improved internalization efficiency as compared with uncoated Si nanorods. To elucidate the molecular mechanism of the... More

关键词

ATXN2; cancer; cell membrane proteins; efficiency; internalization; silicon nanorod