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Response of Sulfolobus solfataricus Dpo4 polymerase in vitro to a DNA G-quadruplex.

Mutagenesis. 2019; 
Berroyer A, Alvarado G, Larson ED.
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Biochemicals … The supernatant was clarified by centrifugation for 30ámin at 11á180 x g. One millilitre of Ni++ agarose (Genscript, Piscataway, NJ, USA) was added to the clarified supernatant and allowed to mix for 30ámin at 4░C. Protein-bound Ni++ resin was collected on a column and the … Get A Quote

摘要

Repetitive DNA sequences support the formation of structures that can interrupt replication and repair, leading to breaks and mutagenesis. One particularly stable structure is G-quadruplex (G4) DNA, which is four-stranded and formed from tandemly repetitive guanine bases. When folded within a template, G4 interferes with DNA synthesis. Similar to non-duplex structures, DNA base lesions can also halt an advancing replication fork, but the Y-family polymerases solve this problem by bypassing the damage. In order to better understand how guanine-rich DNA is replicated, we have investigated the activity of the model Y-family polymerase, Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), on guanine-rich templates ... More

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