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A vesicular stomatitis pseudovirus expressing the surface glycoproteins of influenza A virus.

Arch Virol. 2014; 
Cheresiz SV, Kononova AA, Razumova YV, Dubich TS, Chepurnov AA, Kushch AA, Davey R, Pokrovsky AG.
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ORF cDNA Clones/MolecularCloud … Materials and methods. Plasmids. HA ORF from influenza virus A/Egypt/N04395/2009 (GenBank accession no. CY041941) preceded by a Kozak ribosome initiation consensus sequence was synthesized by GenScript and was subcloned into the pcDNA3 vector … Get A Quote

摘要

Pseudotyped viruses bearing the glycoprotein(s) of a donor virus over the nucleocapsid core of a surrogate virus are widely used as safe substitutes for infectious virus in virology studies. Retroviral particles pseudotyped with influenza A virus glycoproteins have been used recently for the study of influenza hemagglutinin and neuraminidase-dependent processes. Here, we report the development of vesicular-stomatitis-virus-based pseudotypes bearing the glycoproteins of influenza A virus. We show that pseudotypes bearing the hemagglutinin and neuraminidase of H5N1 influenza A virus mimic the wild-type virus in neutralization assays and sensitivity to entry inhibitors. We demonstrate the requirement of NA for the... More

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