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Identification and evaluation of strain B37 of Bacillus subtilis antagonistic to sapstain fungi on poplar wood.

ScientificWorldJournal. 2014; 
Zhang X, Zhao G, Li D, Li S, Hong Q.
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Biochemicals … with the following thermal program: an initial denaturation step (94°C, 5 min) followed by 30 cycles of denaturation (95°C, 30 s), annealing (54°C, 30 s), and extension (72°C, 60 s). After a final extension step (72°C, 20 min), samples were kept at 4°C. The GenScript Co., Ltd … Get A Quote

摘要

Devaluation of poplar products by sapstain accounts for huge and unpredictable losses each year in China. We had isolated four poplar sapstain fungi, Ceratocystis adiposa Hz91, Lasiodiplodia theobromae YM0737, L. theobromae Fx46, and Fusarium sp. YM05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties. After being experimented with agar plates, wood chips, and enzyme activities, strain B37 was identified as the best poplar sapstain biocontrol bacterium. The strain B37 was identified as Bacillus subtilis using sequences of the 16S rRNA gene, physiological biochemical, and morphological characteristics.

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