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Molecular Biology Tools> | The identified proteins were further screened for the presence of MAPK-specific phosphorylation motif by using GPS 3.0–Kinase-specific Phosphorylation Site Prediction69. Finally, Wolf Psort prediction tool (http://www.genscript.com/wolf-psort.html) was used for the prediction of protein subcellular localization. | Get A Quote |
Arabidopsis MPK4 and MPK6 are implicated in different signalling pathways responding to diverse external stimuli. This was recently correlated with transcriptomic profiles of Arabidopsis mpk4 and mpk6 mutants, and thus it should be reflected also on the level of constitutive proteomes. Therefore, we performed a shot gun comparative proteomic analysis of Arabidopsis mpk4 and mpk6 mutant roots. We have used bioinformatic tools and propose several new proteins as putative MPK4 and MPK6 phosphorylation targets. Among these proteins in the mpk6 mutant were important modulators of development such as CDC48A and phospholipase D alpha 1. In the case of the mpk4 mutant transcriptional reprogramming might be mediated by ... More