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Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation.

Int J Mol Sci. 2019; 
Guo H, Guo H, Zhang L, Fan Y, Fan Y, Tang Z, Zeng F.
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Biochemicals There, we used wolfpsort (http:// www.genscript.com/psort/wolf_psort.html) a subcellular localization predication soft to predict subcellular localization Get A Quote

摘要

The somatic embryogenesis (SE) process of plants, as one of the typical responses to abiotic stresses with hormone, occurs through the dynamic expression of different proteins that constitute a complex regulatory network in biological activities and promotes plant totipotency. Plant SE includes two critical stages: primary embryogenic calli redifferentiation and somatic embryos development initiation, which leads to totipotency. The isobaric labels tandem mass tags (TMT) large-scale and quantitative proteomics technique was used to identify the dynamic protein expression changes in nonembryogenic calli (NEC), primary embryogenic calli (PEC) and globular embryos (GEs) of cotton. A total of 9369 proteins (6730 qu... More

关键词

concerted network; cotton; molecular basis; quantitative proteomics; regulation and metabolism; somatic embryogenesis; transdifferentiation