Products/Services Used | Details | Operation |
---|---|---|
Nucleic Acid Purification & Analysis> | Protein size and amount Sample purity Western blot preparation SDS (denaturing) PAGE Gradient gels Single-concentration gels Economy and premium line Fits most popular electrophoresis chambers Format: 8 × 10 cm https://www.genscript. com/page-gels.html | Get A Quote |
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit composition, track post-translational modifications, and verify identity and homogeneity of protein samples. It can also serve to purify proteins for use in further applications. In polyacrylamide gel electrophoresis, proteins migrate in response to an electrical field through pores in a polyacrylamide gel matrix; pore size decreases with increasing acrylamide concentration. Nondenaturing or "native" electrophoresis-i.e., electrophoresis in the absence of denaturants such as detergents and urea-is an often-overlooked technique for determining... More