至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

One-Dimensional Electrophoresis Using Nondenaturing Conditions.

Curr Protoc Protein Sci. 2018; 
Gallagher SR.
Products/Services Used Details Operation
Nucleic Acid Purification & Analysis Protein size and amount Sample purity Western blot preparation SDS (denaturing) PAGE Gradient gels Single-concentration gels Economy and premium line Fits most popular electrophoresis chambers Format: 8 × 10 cm https://www.genscript. com/page-gels.html Get A Quote

摘要

Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit composition, track post-translational modifications, and verify identity and homogeneity of protein samples. It can also serve to purify proteins for use in further applications. In polyacrylamide gel electrophoresis, proteins migrate in response to an electrical field through pores in a polyacrylamide gel matrix; pore size decreases with increasing acrylamide concentration. Nondenaturing or "native" electrophoresis-i.e., electrophoresis in the absence of denaturants such as detergents and urea-is an often-overlooked technique for determining... More

关键词

electrophoresis; native PAGE; native electrophoresis; polyacrylamide; protein; separation