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Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation.

Fish Shellfish Immunol. 2019; 
Xia Y, He K, Jiao L, Geng Y, Zhang D.
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Nucleic Acid Purification & Analysis … The recombinant protein was purified using Ni-NTA Resin (GenScript, Nanjing, China) according to the supplier's protocol Protein was examined by SDS-PAGE on a 12% gel and visualized with Coomassie brilliant blue R250 … Get A Quote

摘要

Protein enhancer of sevenless 2B, E(sev)2B, is a key adapter protein in the Ras/MAPK signaling pathway which has been reported to be involved in innate immunity. In this study, the gene that encodes AsE(sev)2B was isolated from A. sinica. It was found to contain a 636 bp open reading frame encoding 211 amino acids with a calculated molecular mass of 24.357 kDa and a predicted isoelectric point of 5.39. The predicted protein contains a N-terminal Src homology 3 domain (SH3), a central Src homology 2 domain (SH2), and a C-terminal Src homology 3 domain (SH3). Homology analysis revealed that AsE(sev)2B shares 49%-95% identity with E(sev)2B homologs from other species. In this study, the expression pattern and lo... More

关键词

Artemia sinica; Bacterial stimulation; Development; E(sev)2B; Expression analysis