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Biochemicals> | … Linoleic acid, linolenic acid, arachidonic acid and IPTG were purchased from Sigma Aldrich (St Louis, MO, USA) Maker and NTA agarose columns were purchased from GenScript (Nanjing, China) All other chemicals used were of standard reagent grade … | Get A Quote |
In this study, the bacterial lipoxygenase (LOX) gene from Pseudomonas aeruginosa ATCC27853 (pse-LOX) was cloned, sequenced and heterologous expressed in Escherichia coli by auto-induction expression strategy. Production of the recombinant pse-LOX (pse-rLOX) gene up to 23,850 U/mL (264 mg pure protein/L bacterial culture fluid) was observed in the end of this process. To the best of our knowledge, this is the first attempt to manipulate LOX heterologous expression process using auto-induction expression approach, and it is the highest production of recombinant LOX compared with other reports. Subsequently, the resulted pse-rLOX was proved to efficiently degrade triphenylmethane dyes such as malachite green, br... More