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The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets.

Viruses. 2019; 
Wang G,, Liang R,, Liu Z,, Shen Z,, Shi J,, Shi Y,, Deng F,, Xiao S,, Fu ZF,,, Peng G,,.
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DNA Sequencing … Nanjing, Jiangsu, China) The natural complete genome of TGEV WH-1 was determined by sequencing (GenScript, Nanjing, China) the overlapping PCR products cloned into the corresponding vectors in triplicate Compared with … Get A Quote

摘要

Transmissible gastroenteritis virus (TGEV) is the etiologic agent of transmissible gastroenteritis in pigs, and the N-terminal domain of TGEV spike protein is generally recognized as both the virulence determinant and enteric tropism determinant. Here, we assembled a full-length infectious cDNA clone of TGEV in a bacterial artificial chromosome. Using a novel approach, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems efficiently and rapidly rescued another recombinant virus with a 224-amino-acid deletion in the N-terminal domain of the TGEV Spike gene (S_NTD224), which is analogous to the N-terminal domain of porcine respiratory coronavirus. S_NTD2... More

关键词

CRISPR/Cas9; enteric tropism; reverse genetics; spike gene; transmissible gastroenteritis virus